Premium
tRNA import into yeast mitochondria is regulated by the ubiquitin‐proteasome system
Author(s) -
Brandina Irina,
Smirnov Alexandre,
Kolesnikova Olga,
Entelis Nina,
Krasheninnikov Igor A.,
Martin Robert P.,
Tarassov Ivan
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.013
Subject(s) - saccharomyces cerevisiae , proteasome , yeast , mitochondrion , transfer rna , ubiquitin , cytosol , biochemistry , microbiology and biotechnology , lysine , biology , chemistry , rna , gene , amino acid , enzyme
In Saccharomyces cerevisiae , one of two cytosolic lysine‐tRNAs is partially imported into mitochondria. We demonstrate that three components of the ubiquitin/26S proteasome system (UPS), Rpn13p, Rpn8p and Doa1p interact with the imported tRNA and with the essential factor of its mitochondrial targeting, pre‐Msk1p. Genetic and biochemical assays demonstrate that UPS plays a dual regulatory role, since the overall inhibition of cellular proteasome activity reduces tRNA import, while specific depletion of Rpn13p or Doa1p increases it. This result suggests a functional link between UPS and tRNA mitochondrial import in yeast and indicates on the existence of negative and positive import regulators.