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Cholesterol is more susceptible to oxidation than linoleates in cultured cells under oxidative stress induced by selenium deficiency and free radicals
Author(s) -
Saito Yoshiro,
Yoshida Yasukazu,
Niki Etsuo
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.010
Subject(s) - radical , oxidative stress , selenium , chemistry , oxidative phosphorylation , selenium deficiency , biochemistry , catalase , organic chemistry , glutathione peroxidase
Polyunsaturated fatty acids and their esters are known to be susceptible to free‐radical mediated oxidation, while cholesterol is more resistant to oxidation. The present study focused on the relative susceptibilities of linoleates and cholesterol in Jurkat cells under oxidative stress induced by selenium deficiency and free radical insult, as assessed by total hydroxyoctadecadienoic acids (tHODE) and total 7‐hydroxycholesterol (t7‐OHCh) measured after reduction and saponification. It was observed that the levels of tHODE and t7‐OHCh significantly increased by both oxidative insults. The increased amounts of t7‐OHCh were higher than those of tHODE in both selenium‐deficient and free radical‐treated cells. These results suggest that, in contrast to plasma oxidation where cholesterol is much more resistant to oxidation than linoleates, cellular cholesterol is more susceptible to oxidation than cellular linoleates.