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Tumor necrosis factor‐α exerts pro‐myogenic action in C2C12 myoblasts via sphingosine kinase/S1P 2 signaling
Author(s) -
Donati Chiara,
Nincheri Paola,
Cencetti Francesca,
Rapizzi Elena,
Farnararo Marta,
Bruni Paola
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.007
Subject(s) - myogenesis , sphingosine kinase , c2c12 , sphingosine kinase 1 , sphingosine , tumor necrosis factor alpha , myocyte , microbiology and biotechnology , sphingosine 1 phosphate , cytokine , biology , signal transduction , kinase , cancer research , endocrinology , medicine , immunology , biochemistry , receptor
In this study, we report that low doses of tumor necrosis factor‐α (TNFα) promote myogenesis in C2C12 myoblasts. Moreover, the cytokine increased sphingosine kinase (SphK) activity and induced SphK1 translocation to membranes. The inhibition of SphK functionality by various approaches abrogated the pro‐myogenic effect of TNFα. Moreover, silencing of S1P 2 impaired the positive action of TNFα on myogenesis. These results represent the first evidence that SphK/S1P 2 axis is required for the regulation of myogenesis by TNFα. In view of the physiological role of TNFα in muscle regeneration, the present finding reinforces the notion that SphK/S1P 2 signaling is critically implicated in myogenesis.