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Single exposure of human fibroblasts (WI‐38) to a sub‐cytotoxic dose of UVB induces premature senescence
Author(s) -
Straface Elisabetta,
Vona Rosa,
Ascione Barbara,
Matarrese Paola,
Strudthoff Tiziana,
Franconi Flavia,
Malorni Walter
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.006
Subject(s) - photoaging , senescence , cytotoxic t cell , microbiology and biotechnology , chemistry , in vitro , premature aging , fibroblast , phenotype , cancer research , biology , biochemistry , genetics , gene
In this work, we present a new model of stress‐induced premature senescence obtained by exposing human fibroblasts (WI‐38) at early passages (passages 2–4) to a single sub‐cytotoxic dose of UVB (200 mJ/cm 2 ). We show that this treatment leads to the appearance of several biomarkers of senescence such as enlarged and flattened cell morphology, the presence of nuclear heterochromatic foci and β‐galactosidase activity. Furthermore, we demonstrate that a mild ROS production and p53 activation are upstream events required for the induction of premature senescence. Our method represents an alternative in vitro model in photoaging research and could be used to test potential anti‐photoaging compounds.