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peg10 , an imprinted gene, plays a crucial role in adipocyte differentiation
Author(s) -
Hishida Tomoaki,
Naito Kumiko,
Osada Shigehiro,
Nishizuka Makoto,
Imagawa Masayoshi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.074
Subject(s) - adipogenesis , gene knockdown , adipocyte , genomic imprinting , rna interference , biology , gene , microbiology and biotechnology , cellular differentiation , lipid droplet , gene expression , genetics , biochemistry , rna , adipose tissue , dna methylation
An imprinted gene, paternally expressed gene ( peg ) 10 , was isolated as one of the genes expressed early in adipogenesis. The expression of peg10 was elevated after the addition of inducers, and was detected in adipocyte differentiable 3T3‐L1 cells, but not observed in the non‐adipogenic cell line NIH‐3T3. Moreover, the knockdown of peg10 by RNA interference (RNAi) inhibited the differentiation of 3T3‐L1 cells into lipid‐laden adipocytes. Interestingly, peg10 RNAi‐treatment reduced the expressions of C/EBPβ and C/EBPδ, and inhibited mitotic clonal expansion. These findings strongly indicate that peg10 plays a crucial role at the immediate early stage of adipocyte differentiation.