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The GluCre‐ROSA26EYFP mouse: A new model for easy identification of living pancreatic α‐cells
Author(s) -
Quoix Nicolas,
Cheng-Xue Rui,
Guiot Yves,
Herrera Pedro L.,
Henquin Jean-Claude,
Gilon Patrick
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.068
Subject(s) - tolbutamide , diazoxide , alpha cell , glucagon , alpha (finance) , endocrinology , pancreatic islets , medicine , secretion , chemistry , insulin , arginine , biology , islet , beta cell , biochemistry , amino acid , construct validity , nursing , patient satisfaction
The control of glucagon secretion by pancreatic α‐cells is poorly understood, largely because of the difficulty to recognize living α‐cells. We describe a new mouse model, referred to as GluCre‐ROSA26EYFP (or GYY), allowing easy α‐cell identification because of specific expression of EYFP. GYY mice displayed normal glycemic control during a fasting/refeeding test or intraperitoneal insulin injection. Glucagon secretion by isolated islets was normally inhibited by glucose and stimulated by adrenaline. [Ca 2+ ] c responses to arginine, adrenaline, diazoxide and tolbutamide, were similar in GYY and control mice. Hence, this new mouse model is a reliable and powerful tool to specifically study α‐cells.
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