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The antioxidative function of eicosapentaenoic acid in a marine bacterium, Shewanella marinintestina IK‐1
Author(s) -
Nishida Takanori,
Morita Naoki,
Yano Yutaka,
Orikasa Yoshitake,
Okuyama Hidetoshi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.065
Subject(s) - eicosapentaenoic acid , chemistry , shewanella , bacteria , microbiology and biotechnology , function (biology) , biochemistry , biology , fatty acid , polyunsaturated fatty acid , genetics
When the eicosapentaenoic acid (EPA)‐deficient mutant strain IK‐1Δ8 of the marine EPA‐producing Shewanella marinintestina IK‐1 was treated with various concentrations of hydrogen peroxide (H 2 O 2 ), its colony‐forming ability decreased more than that of the wild type. Protein carbonylation, induced by treating cells with 0.01 mM H 2 O 2 under bacteriostatic conditions, was enhanced only in cells lacking EPA. The amount of cells recovered from the cultures was decreased more significantly by the presence of H 2 O 2 for cells lacking EPA than for those producing EPA. Treatment of the cells with 0.1 mM H 2 O 2 resulted in much lower intracellular concentrations of H 2 O 2 being consistently detected in cells with EPA than in those without EPA. These results suggest that cellular EPA can directly protect cells against oxidative damage by shielding the entry of exogenously added H 2 O 2 in S. marinintestina IK‐1.