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Expression and selective up‐regulation of toxin‐related mono ADP‐ribosyltransferases by pathogen‐associated molecular patterns in alveolar epithelial cells
Author(s) -
Balducci Enrico,
Micossi Luigi G.,
Soldaini Elisabetta,
Rappuoli Rino
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.061
Subject(s) - flagellin , peptidoglycan , tlr2 , lipoteichoic acid , innate immune system , tlr4 , lipopolysaccharide , biology , tlr5 , toll like receptor , a549 cell , microbiology and biotechnology , pathogen associated molecular pattern , receptor , immune system , gene , cell , biochemistry , pattern recognition receptor , bacteria , immunology , staphylococcus aureus , genetics
Mono ADP‐ribosyltransferases (ARTs) are a family of enzymes related to bacterial toxins that possess adenosine diphosphate ribosyltransferase activity. We have assessed that A549 constitutively expressed ART1 on the cell surface and shown that lipotheicoic acid (LTA) and flagellin, but not lipopolysaccharide (LPS), peptidoglycan (PG) and poly (I:C), up‐regulate ART1 in a time and dose dependent manner. These agonists did not alter the expression of ART3 and ART5 genes. Indeed, LTA and flagellin stimulation increased the level of ART1 protein and transcript while ART4 gene was activated after stimulation of cells with LPS, LTA, PAM and PG via TLR2 and TLR4 receptors. These results show that human ARTs possess a differential capacity to respond to bacteria cell wall components and might play a crucial role in innate immune response in airways.