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Bioinformatic analysis of post‐transcriptional regulation by uORF in human and mouse
Author(s) -
Matsui Motomu,
Yachie Nozomu,
Okada Yuki,
Saito Rintaro,
Tomita Masaru
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.057
Subject(s) - upstream open reading frame , biology , rna , gene , messenger rna , gene expression , post transcriptional regulation , translation (biology) , ribosome , ribosome profiling , genetics , open reading frame , microbiology and biotechnology , computational biology , peptide sequence
RNA decay is thought to exert an important influence on gene expression by maintaining a steady‐state level of transcripts and/or by eliminating aberrant transcripts. However, the sequence elements which control such processes have not been determined. Upstream open reading frames (uORFs) in the transcripts of several genes are reported to control translational initiation by stalling ribosomes and thereby promote RNA decay. We therefore performed bioinformatic analysis of the tissue‐wide expression profiles and mRNA half‐life of transcripts containing uORFs in humans and mice to assess the relationship between RNA decay and the presence of uORFs in transcripts. The expression levels of transcripts containing uORF were markedly lower than those not containing uORF. Moreover, the half‐life of the uORF‐containing transcripts was also shorter. These results suggest that uORFs are sequence elements that down‐regulate RNA transcripts via RNA decay mechanisms.