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Presence of robust circadian clock oscillation under constitutive over‐expression of mCry1 in rat‐1 fibroblasts
Author(s) -
Yamanaka Iori,
Koinuma Satoshi,
Shigeyoshi Yasufumi,
Uchiyama Yasuo,
Yagita Kazuhiro
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.053
Subject(s) - circadian rhythm , circadian clock , microbiology and biotechnology , oscillation (cell signaling) , luciferase , biology , gene expression , period (music) , reporter gene , gene , endocrinology , transfection , genetics , physics , acoustics
In mammals, mCRY proteins are essential and are major negative elements in circadian feedback loops. In this study, robust circadian clock oscillation was present even under conditions with constitutive over‐expression of mCry1 in rat‐1 cells. Rat‐1 cells were produced to stably express mPer2 promoter‐driven luciferase reporter, in which mCry1 was overexpressed under a tetracycline‐dependent gene expression (Tet‐On) system. Using these cells, we show that circadian clock oscillations in rat‐1 fibroblasts persist when the mCRY1 protein constitutively accumulates in the nuclei.