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Mesenchymal stem cells attenuate cardiac fibroblast proliferation and collagen synthesis through paracrine actions
Author(s) -
Ohnishi Shunsuke,
Sumiyoshi Hideaki,
Kitamura Soichiro,
Nagaya Noritoshi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.028
Subject(s) - mesenchymal stem cell , paracrine signalling , myocardin , microbiology and biotechnology , downregulation and upregulation , fibroblast , cancer research , chemistry , elastin , biology , immunology , gene expression , gene , biochemistry , in vitro , receptor , genetics , serum response factor
Mesenchymal stem cells (MSC) transplantation has been shown to decrease fibrosis in the heart; however, whether MSC directly influence the function of cardiac fibroblasts (CFB) remains unknown. MSC‐conditioned medium significantly attenuated proliferation of CFB compared with CFB‐conditioned medium. MSC‐conditioned medium upregulated antiproliferation‐related genes such as elastin, myocardin and DNA‐damage inducible transcript 3, whereas CFB‐conditioned medium upregulated proliferation‐related genes such as alpha‐2‐macrogrobulin and v‐kit Hardy–Zuckerman 4 feline sarcoma viral oncogene homolog. MSC‐conditioned medium significantly downregulated type I and III collagen expression, and significantly suppressed type III collagen promoter activity. MSC may exert paracrine anti‐fibrotic effects at least in part through regulation of CFB proliferation and collagen synthesis.