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Cloning and characterization of microRNAs from Brassica napus
Author(s) -
Wang Lei,
Wang Ming-Bo,
Tu Jin-Xing,
Helliwell Christopher A.,
Waterhouse Peter M.,
Dennis Elizabeth S.,
Fu Ting-Dong,
Fan Yun-Liu
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.07.010
Subject(s) - brassica , biology , cloning (programming) , microrna , arabidopsis , genetics , rna , genome , small rna , gene , ploidy , arabidopsis thaliana , computational biology , botany , mutant , computer science , programming language
A library containing approximately 40,000 small RNA sequences was constructed for Brassica napus . Analysis of 3025 sequences obtained from this library resulted in the identification of 11 conserved miRNA families, which were validated by secondary structure prediction using surrounding sequences in the Brassica genome. Two 21 nt small RNA sequences reside within the arm of a pre‐miRNA like stem–loop structure, making them likely candidates for novel non‐conserved miRNAs in B. napus . Most of the conserved miRNAs were expressed at similar levels in a F1 hybrid B. napus line and its four double haploid progeny that showed marked variations in phenotypes, but many were differentially expressed between B. napus and Arabidopsis. The miR169 family was expressed at high levels in young leaves and stems, but was undetectable in roots and mature leaves, suggesting that miR169 expression is developmentally regulated in B. napus .