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H1.X with different properties from other linker histones is required for mitotic progression
Author(s) -
Takata Hideaki,
Matsunaga Sachihiro,
Morimoto Akihiro,
Ono-Maniwa Rika,
Uchiyama Susumu,
Fukui Kiichi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.06.076
Subject(s) - chromatin , mitosis , interphase , nucleolus , photobleaching , histone h1 , histone , biology , chromosome , microbiology and biotechnology , genetics , chromosome segregation , linker , biophysics , cytoplasm , fluorescence , physics , gene , optics , computer science , operating system
We report here the characterization of H1.X, a human histone H1 subtype. We demonstrate that H1.X accumulates in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. In addition, the results of fluorescence recovery after photobleaching indicate that the exchange of H1.X on and off chromatin is faster than that of the other H1 subtypes. Furthermore, RNA interference experiments reveal that H1.X is required for chromosome alignment and segregation. Our results suggest that H1.X has important functions in mitotic progression, which are different from those of the other H1 subtypes.