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Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose‐6‐phosphate dehydrogenase
Author(s) -
Nicolas Cécile,
Kiefer Patrick,
Letisse Fabien,
Krömer Jens,
Massou Stéphane,
Soucaille Philippe,
Wittmann Christoph,
Lindley Nic D.,
Portais Jean-Charles
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.06.066
Subject(s) - escherichia coli , gene , dehydrogenase , biochemistry , chemistry , metabolism , gene expression , carbohydrate metabolism , biology , enzyme
The deletion of the zwf gene encoding G6PDH activity led to restructuring of the carbon flux through central metabolism in Escherichia coli , though over‐expression of this gene had only minor consequences for overall carbon flux. The modified carbon flux seen in the zwf deletion mutant enabled alternative routes of anabolic precursor formation and an adequate supply of NADPH synthesis via a modified TCA cycle to be generated so as to sustain growth rates comparable to the WT.