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p53 mediates interstitial cystitis antiproliferative factor (APF)‐induced growth inhibition of human urothelial cells
Author(s) -
Kim Jayoung,
Keay Susan K.,
Dimitrakov Jordan D.,
Freeman Michael R.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.06.058
Subject(s) - interstitial cystitis , urothelial cell , cancer research , blockade , ectopic expression , growth factor , urinary system , urinary bladder , medicine , urothelium , chemistry , gene , receptor , biochemistry
Antiproliferative factor (APF) is a sialoglycopeptide elevated in the urine of patients with interstitial cystitis, a urinary bladder disorder of unknown etiology that is characterized by chronic pelvic pain. The present study was directed toward uncovering a pathway through which APF signals. Treatment of human urothelial cells with native APF resulted in growth inhibition accompanied by blockade of cell cycle transit and increased p53. Reduced expression of p53 by RNA interference diminished, while ectopic expression of p53 mimicked, the effects of APF. These are the first findings implicating the network of p53 target genes in urothelial defects associated with interstitial cystitis.