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Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB‐1
Author(s) -
Suzuki Takeyuki,
Okamura Yoshiko,
Arakaki Atsushi,
Takeyama Haruko,
Matsunaga Tadashi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.06.047
Subject(s) - magnetotactic bacteria , ferrous , cytoplasm , transposon mutagenesis , mutant , atpase , biology , biochemistry , mutagenesis , transposable element , strain (injury) , gene , bacteria , microbiology and biotechnology , biophysics , chemistry , enzyme , genetics , organic chemistry , anatomy
A non‐magnetic mutant of Magnetospirillum magneticum AMB‐1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild‐type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild‐type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB‐1.