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Cell‐surface H + ‐ATP synthase as a potential molecular target for anti‐obesity drugs
Author(s) -
Arakaki Naokatu,
Kita Toshiyuki,
Shibata Hirofumi,
Higuti Tomihiko
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.06.041
Subject(s) - atp synthase , cytosol , cell , chemistry , biochemistry , v atpase , adipocyte , enzyme , biology , atpase , adipose tissue
Here we show that the cell‐surface expression of the alpha subunit of H + ‐ATP synthase is markedly increased during adipocyte differentiation. Treatment of differentiated adipocytes with small molecule inhibitors of H + ‐ATP synthase or antibodies against alpha and beta subunits of H + ‐ATP synthase leads to a decrease in cytosolic lipid droplet accumulation. Apolipoprotein A‐I, which has been shown to bind to the ectopic β‐chain of H + ‐ATP synthase and inhibit the activity of cell‐surface H + ‐ATP synthase, also was found to inhibit cytosolic lipid accumulation. These results suggest that the cell‐surface H + ‐ATP synthase has a previously unsuspected role in lipid metabolism in adipocytes.