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Identification of trehalose dimycolate (cord factor) in Mycobacterium leprae
Author(s) -
Kai Masanori,
Fujita Yukiko,
Maeda Yumi,
Nakata Noboru,
Izumi Shinzo,
Yano Ikuya,
Makino Masahiko
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.06.029
Subject(s) - glycolipid , mycobacterium leprae , trisaccharide , chemistry , bacteria , armadillo , trehalose , microbiology and biotechnology , biochemistry , biology , leprosy , immunology , genetics
Glycolipids of Mycobacterium leprae obtained from armadillo tissue nodules infected with the bacteria were analyzed. Mass spectrometric analysis of the glycolipids indicated the presence of trehalose 6,6′‐dimycolate (TDM) together with trehalose 6‐monomycolate (TMM) and phenolic glycolipid‐I (PGL‐I). The analysis showed that M. leprae ‐derived TDM and TMM possessed both α‐ and keto‐mycolates centering at C78 in the former and at C81 or 83 in the latter subclasses, respectively. For the first time, MALDI‐TOF mass analyses showed the presence of TDM in M. leprae .