Clusterin is associated with spontaneous breast cancer in TA2 mice

Two‐dimensional electrophoresis and Matrix‐assisted laser desorption ionization‐time of flight‐time of mass spectrometry were used to detect the differentially expressed proteins in serum of tientsin albinao 2 mice with spontaneous breast cancer, normal tientsin albinao 2 mice and tientsin albinao 1 mice. Only nuclear clusterin (n‐CLU) was expressed in tientsin albinao 1. Immunohistochemistry and western blot validated that n‐CLU was present in normal tientsin albinao 2 and tientsin albinao 1 mammary epithelium, and secretory clusterin expressed in the cytoplasm of normal tientsin albinao 2 mammary epithelium and spontaneous breast cancer. n‐CLU may play an important role in tientsin albinao 2 spontaneous breast cancer initiation and development.