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Calcium‐mediated, purinergic stimulation and polarized localization of calcium‐sensitive adenylyl cyclase isoforms in human airway epithelia
Author(s) -
Nlend Marie-Christine,
Schmid Andreas,
Sutto Zoltan,
Ransford George A.,
Conner Gregory E.,
Fregien Nevis,
Salathe Matthias
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.06.015
Subject(s) - purinergic receptor , adenylyl cyclase , stimulation , microbiology and biotechnology , calcium , biology , chemistry , medicine , endocrinology , extracellular
Purinergic stimulation of human airway epithelia results in a prolonged increase in ciliary beat frequency that depends on calcium‐mediated cAMP production [Lieb, T., Wijkstrom Frei, C., Frohock, J.I., Bookman, R.J. and Salathe, M. (2002) Prolonged increase in ciliary beat frequency after short‐term purinergic stimulation in human airway epithelial cells. J. Physiol. (Lond.) 538, 633–646]. Here, fully differentiated human airway epithelial cells in culture are shown to express calcium‐stimulated transmembrane adenylyl cyclase (tmAC) isoforms (types 1, 3, and 8) by reverse transcription polymerase chain reaction. Immunohistochemistry of tracheal sections and fully differentiated airway epithelial cell cultures revealed polarized expression of these tmACs, with types 1 and 8 localized to the apical membrane and thus at the position required for ciliary regulation. Real‐time, ciliated‐cell specific cAMP production by tmACs upon apical, purinergic stimulation with UTP was confirmed using fluorescent energy resonance transfer between fluorescently tagged PKA subunits.