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ELOVL2 overexpression enhances triacylglycerol synthesis in 3T3‐L1 and F442A cells
Author(s) -
Kobayashi Tsutomu,
Zadravec Damir,
Jacobsson Anders
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.05.081
Subject(s) - arachidonic acid , eicosapentaenoic acid , diacylglycerol kinase , lipid droplet , biochemistry , acyltransferase , fatty acid , monoacylglycerol lipase , chemistry , microbiology and biotechnology , gene , biology , polyunsaturated fatty acid , protein kinase c , enzyme , receptor , endocannabinoid system
Elongation of very long‐chain fatty acids (ELOVL) members were overexpressed in two preadipocyte cell lines, ELOVL2 and ELOVL3 in 3T3‐L1 cells, and ELOVL1–3 in F442A cells. Cells overexpressing ELOVL2, whose preferred substrates are arachidonic acid (AA, C20:4 n −6) and eicosapentaenoic acid (EPA, C20:5 n −3), showed an enhanced triacylglycerol (TAG) synthesis and subsequent accumulation of lipid droplets. Incorporation of fatty acid (FA) but not of glucose into TAG was enhanced by ELOVL2‐overexpression. Two lipogenic genes encoding diacylglycerol acyltransferase‐2 (DGAT2) and fatty acid‐binding protein‐4 (FABP4, aP2) were induced in ELOVL2‐overexpressing cells, whereas no such effect was seen on the fatty acid synthase (FAS) gene.