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Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation
Author(s) -
Adamski Danièle,
Mayol Jean-François,
Platet Nadine,
Berger François,
Hérodin Francis,
Wion Didier
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.05.073
Subject(s) - side population , c2c12 , stem cell , staining , efflux , population , cell , microbiology and biotechnology , chemistry , biology , cancer stem cell , biochemistry , myocyte , medicine , genetics , environmental health , myogenesis
Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation of data.