Premium
Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line
Author(s) -
Takenouchi Takato,
Iwamaru Yoshifumi,
Imamura Morikazu,
Kato Nobuko,
Sugama Shuei,
Fujita Masayo,
Hashimoto Makoto,
Sato Mitsuru,
Okada Hiroyuki,
Yokoyama Takashi,
Mohri Shirou,
Kitani Hiroshi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.05.057
Subject(s) - scrapie , biology , infectivity , receptor , cell culture , downregulation and upregulation , pathogenesis , cell , in vitro , microglia , programmed cell death , virology , microbiology and biotechnology , immunology , gene , inflammation , virus , disease , apoptosis , prion protein , pathology , genetics , medicine
We recently established mouse microglial cells persistently infected with mouse‐adapted scrapie ME7 (ScMG20/ME7) for in vitro study of prion pathogenesis. Here, we found that ScMG20/ME7 cells were hypersensitive to P2X7 receptor agonists, as demonstrated by sustained Ca 2+ influx, membrane pore formation, cell death, and interleukin‐1β release. P2X7 mRNA expression was upregulated in these cells, and also in scrapie‐infected mice brains. Treatment with pentosan polysulfate eliminated the infectivity and disease‐related forms of prion protein from ScMG20/ME7 cell cultures, however, hypersensitivity of P2X7 receptors remained. These results suggest that prion infections may strongly affect the P2X7 receptor system in mouse microglial cells.