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Genome‐wide screening of genes affecting glycogen metabolism in Escherichia coli K‐12
Author(s) -
Eydallin Gustavo,
Viale Alejandro M.,
Morán-Zorzano María Teresa,
Muñoz Francisco José,
Montero Manuel,
Baroja-Fernández Edurne,
Pozueta-Romero Javier
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.05.044
Subject(s) - glycogen , mutant , gene , escherichia coli , biology , biochemistry , glycogen synthase , phenotype
A systematic and comprehensive gene‐disrupted mutant collection of E. coli K‐12 was used to identify genes whose deletions affect glycogen accumulation. Of the 3985 non‐essential gene mutants of the collection, 35 displayed a glycogen‐excess phenotype, whereas 30 displayed either glycogen‐less or glycogen‐deficient phenotypes. The genes whose deletions affect glycogen accumulation were classified into various functional categories, including energy production, envelope composition and integrity, protein translation and stability, transport of inorganic ions and nucleotides, and metabolism of carbohydrates and amino acids. The overall data indicate that glycogen metabolism is highly interconnected with a wide variety of cellular processes in E. coli .