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The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei
Author(s) -
Szilvay Géza R.,
Kisko Kaisa,
Serimaa Ritva,
Linder Markus B.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.05.024
Subject(s) - hydrophobin , trichoderma reesei , tetramer , chemistry , biochemistry , enzyme , gene , cellulase
Hydrophobins are small fungal surface active proteins that self‐assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.