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Outgrowing endothelial progenitor‐derived cells display high sensitivity to angiogenesis modulators and delayed senescence
Author(s) -
Ha Jung-Min,
Kim Myoung-Rae,
Oh Ho-Kyun,
Lee Byung Hun,
Ahn Hyun-Young,
Shin Jong-Chul,
Baek Sang Hong,
Joe Young Ae
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.05.010
Subject(s) - vasculogenesis , progenitor cell , angiogenesis , microbiology and biotechnology , umbilical vein , biology , endothelial stem cell , senescence , progenitor , population , immunology , neovascularization , endothelial progenitor cell , stem cell , in vitro , genetics , medicine , environmental health
Outgrowing endothelial progenitor‐derived cells (EPDCs) originate from a novel hierarchy of endothelial progenitor cells. In this study, EPDCs isolated from human cord blood were examined for phenotype and functional features upon aging. Young or aged EPDCs were similar to human umbilical vein endothelial cells (HUVECs), in exhibiting typical endothelial phenotypes. However, EPDCs were more sensitive to angiogenesis inducers or inhibitors in proliferation and migration. In addition, EPDCs underwent senescence markedly slowly with sustained endothelial NO synthase expression and activation, and their ability to undergo capillary morphogenesis was retained throughout longterm culture. Thus, these results suggest that a homogenous population of EPDCs derived from clonogenic expansion may provide an effective vasculogenesis tool.