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Post‐transcriptional regulation of the Brn‐3b transcription factor in differentiating neuroblastoma cells
Author(s) -
Calissano Mattia,
Diss James K.J.,
Latchman David S.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.04.066
Subject(s) - dicer , microrna , three prime untranslated region , messenger rna , transcription factor , untranslated region , neuroblastoma , microbiology and biotechnology , biology , transcription (linguistics) , gene , rna , cell culture , genetics , rna interference , linguistics , philosophy
We show that the 3′UTR of the Brn‐3b mRNA does indeed contain regulatory sequences that mediate mRNA degradation upon serum starvation‐induced differentiation of ND7 neuroblastoma cells. The specific region mediating this effect has been characterized and two different microRNAs that potentially regulate the stability of Brn‐3b have been identified. Moreover we show that Dicer, one of the key enzymes in the production of microRNAs, is strongly up‐regulated in ND7 cells subjected to differentiation.