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Characterisation of the ATP‐dependent phosphofructokinase gene family from Arabidopsis thaliana
Author(s) -
Mustroph Angelika,
Sonnewald Uwe,
Biemelt Sophia
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.04.060
Subject(s) - phosphofructokinase , arabidopsis thaliana , arabidopsis , gene , biology , complementary dna , gene family , gene expression , genetics , homology (biology) , microbiology and biotechnology , biochemistry , glycolysis , enzyme , mutant
Plants possess two different types of phosphofructokinases, an ATP‐dependent (PFK) and a pyrophosphate‐dependent form (PFP). While plant PFPs have been investigated in detail, cDNA clones coding for PFK have not been identified in Arabidopsis thaliana . Searching the A. thaliana genome revealed 11 putative members of a phosphofructokinase gene family. Among those, four sequences showed high homology to the alpha‐ or beta‐subunits of plant PFPs. Seven cDNAs resulted in elevated PFK, but not PFP activity after transient expression in tobacco leaves suggesting that they encode Arabidopsis PFKs. RT‐PCR revealed different tissue‐specific expression of the individual forms. Furthermore, analysis of GFP fusion proteins indicated their presence in different sub‐cellular compartments.