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Protein–protein interactions in carotenoid triggered quenching of phycobilisome fluorescence in Synechocystis sp. PCC 6803
Author(s) -
Rakhimberdieva Marina G.,
Bolychevtseva Yulia V.,
Elanskaya Irina V.,
Karapetyan Navassard V.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.04.056
Subject(s) - phycobilisome , quenching (fluorescence) , photochemistry , chemistry , fluorescence , biophysics , synechocystis , arrhenius equation , yield (engineering) , mutant , activation energy , biochemistry , biology , materials science , cyanobacteria , genetics , physics , quantum mechanics , bacteria , gene , metallurgy
An inquiry into the effect of temperature on carotenoid triggered quenching of phycobilisome (PBS) fluorescence in a photosystem II‐deficient mutant of Synechocystis sp. results in identification of two temperature‐dependent processes: one is responsible for the quenching rate, and one determines the yield of PBS fluorescence. Non‐Arrhenius behavior of the light‐on quenching rate suggests that carotenoid‐absorbed light triggers a process that bears a strong resemblance to soluble protein folding, showing temperature‐dependent enthalpy of activated complex formation. The response of PBS fluorescence yield to hydration changing additives and to passing of the membrane lipid phase transition point indicates that the pool size of PBSs subject to quenching depends on the state of some membrane component.