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Differential membrane perturbation caused by the cell penetrating peptide Tp10 depending on attached cargo
Author(s) -
Bárány-Wallje Elsa,
Gaur Jugnu,
Lundberg Pontus,
Langel Ülo,
Gräslund Astrid
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.04.046
Subject(s) - peptide , membrane , vesicle , biophysics , chemistry , cell penetrating peptide , peptide nucleic acid , cell membrane , calcein , biochemistry , biology
The membrane leakage caused by the cell penetrating peptide Tp10, a variant of transportan, was studied in large unilamellar vesicles with the entrapped fluorophore calcein. The vesicles were composed of zwitterionic 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine. A significant decrease in membrane leakage was found when the 55 kDa streptavidin protein was attached to Tp10. When a 5.4 kDa peptide nucleic acid molecule was attached, the membrane leakage was comparable to that caused by Tp10 alone. The results suggest that direct membrane effects may cause membrane translocation of Tp10 alone and of smaller complexes, whereas these effects do not contribute for larger cargoes.