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Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferon
Author(s) -
Hiraga Nobuhiko,
Imamura Michio,
Tsuge Masataka,
Noguchi Chiemi,
Takahashi Shoichi,
Iwao Eiji,
Fujimoto Yoshifumi,
Abe Hiromi,
Maekawa Toshiro,
Ochi Hidenori,
Tateno Chise,
Yoshizato Katsutoshi,
Sakai Akihito,
Sakai Yoshio,
Honda Masao,
Kaneko Shuichi,
Wakita Takaji,
Chayama Kazuaki
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.04.021
Subject(s) - virology , viremia , genotype , hepatitis c virus , clone (java method) , interferon , biology , virus , hepacivirus , hepatitis c , hepatocyte , immunology , gene , genetics , in vitro
We developed a reverse genetics system of hepatitis C virus (HCV) genotypes 1a and 2a using infectious clones and human hepatocyte chimeric mice. We inoculated cell culture‐produced genotype 2a (JFH‐1) HCV intravenously. We also injected genotype 1a CV‐H77C clone RNA intrahepatically. Mice inoculated with HCV by both procedures developed measurable and transmissible viremia. Interferon (IFN) alpha treatment resulted in greater reduction of genotype 2a HCV levels than genotype 1a, as seen in clinical practice. Genetically engineered HCV infection system should be useful for analysis of the mechanisms of resistance of HCV to IFN and other drugs.