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Transfection and expression of plasmid DNA in plant cells by an arginine‐rich intracellular delivery peptide without protoplast preparation
Author(s) -
Chen Chung-Pin,
Chou Jyh-Ching,
Liu Betty Revon,
Chang Microsugar,
Lee Han-Jung
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.03.076
Subject(s) - transfection , protoplast , gene delivery , intracellular , transduction (biophysics) , plant cell , plasmid , biology , transgene , dna , exogenous dna , microbiology and biotechnology , peptide , gene , biochemistry
The delivery and expression of exogenous genes in plant cells have been of particular interest for plant research and biotechnology. Here, we present results demonstrating a simple DNA transfection system in plants. Short arginine‐rich intracellular delivery peptide, a protein transduction domain, was capable of delivering plasmid DNA into living plant cells non‐covalently. This peptide‐mediated DNA delivery conferred several advantages, such as nuclear targeting, non‐toxic effect, and ease of preparation without protoplast formulation. Thus, this novel technology shall provide a powerful tool to investigate gene function in vivo , and lay the foundation for the production of transgenic plants in future.