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Mechanism of vitamin D 3 ‐induced transcription of phospholipase D1 in HaCat human keratinocytes
Author(s) -
Kikuchi Ryosuke,
Sobue Sayaka,
Murakami Masashi,
Ito Hiromi,
Kimura Ami,
Iwasaki Takashi,
Shibayama Shuji,
Takagi Akira,
Kojima Tetsuhito,
Suzuki Motoshi,
Banno Yoshiko,
Nozawa Yoshinori,
Murate Takashi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.03.073
Subject(s) - hacat , calcitriol receptor , chromatin immunoprecipitation , microbiology and biotechnology , chemistry , immunoprecipitation , promoter , biology , gene expression , gene , biochemistry , in vitro
1α,25‐Dihydroxyvitamin D 3 (VitD 3 ) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD 3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (−260 bp to −246 bp from exon 1). Similar results were obtained by transfecting VitD 3 receptor (VDR) into HEK293 cells, which are originally VitD 3 ‐unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD 3 , VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.