Mechanism of vitamin D 3 ‐induced transcription of phospholipase D1 in HaCat human keratinocytes | Zendy

Kikuchi Ryosuke | Zendy; Sobue Sayaka | Zendy; Murakami Masashi | Zendy; Ito Hiromi | Zendy; Kimura Ami | Zendy; Iwasaki Takashi | Zendy; Shibayama Shuji | Zendy; Takagi Akira | Zendy; Kojima Tetsuhito | Zendy; Suzuki Motoshi | Zendy; Banno Yoshiko | Zendy; Nozawa Yoshinori | Zendy; Murate Takashi | Zendy

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Mechanism of vitamin D 3 ‐induced transcription of phospholipase D1 in HaCat human keratinocytes

Author(s) -

Kikuchi Ryosuke,

Sobue Sayaka,

Murakami Masashi,

Ito Hiromi,

Kimura Ami,

Iwasaki Takashi,

Shibayama Shuji,

Takagi Akira,

Kojima Tetsuhito,

Suzuki Motoshi,

Banno Yoshiko,

Nozawa Yoshinori,

Murate Takashi

Publication year - 2007

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2007.03.073

Subject(s) - hacat , calcitriol receptor , chromatin immunoprecipitation , microbiology and biotechnology , chemistry , immunoprecipitation , promoter , biology , gene expression , gene , biochemistry , in vitro

1α,25‐Dihydroxyvitamin D 3 (VitD 3 ) increases protein and gene expression of phospholipase D1 (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD 3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5′ PLD1 promoter (−260 bp to −246 bp from exon 1). Similar results were obtained by transfecting VitD 3 receptor (VDR) into HEK293 cells, which are originally VitD 3 ‐unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD 3 , VDR and retinoid X receptor α (RXRα) binds to the VDRE and increases PLD1 gene expression in HaCaT cells.

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