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Regulation of the BRCA1 promoter in ovarian surface epithelial cells and ovarian carcinoma cells
Author(s) -
Graves Marcia L.,
Zhou Lixin,
MacDonald Gwen,
Mueller Christopher R.,
Roskelley Calvin D.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.03.072
Subject(s) - creb , ap 1 transcription factor , transcription factor , ovarian carcinoma , cancer research , response element , promoter , transcription (linguistics) , cell culture , biology , microbiology and biotechnology , chemistry , ovarian cancer , gene , gene expression , genetics , cancer , linguistics , philosophy
As BRCA1 expression is often suppressed in sporadic ovarian carcinoma we characterized the regulation of the 231nt proximal ‘L6’ fragment of the BRCA1 promoter in two human ovarian surface epithelial cell and two sporadic ovarian carcinoma cell lines. Two individual regulatory elements within L6, the ‘RIBS’ element and the potential ‘CRE’ element were each necessary, but alone not sufficient for L6 activation in all four cell lines. The latter element showed some affinity for the CREB transcription factor, but cAMP pathway stimulation failed to promote its activation. This element did, however, interact with, and was activated by, c‐Jun and Fra2 which suggests that it can interact with AP1‐like transcription factors and that it may act co‐operatively with RIBS‐binding factors to regulate BRCA1 transcription in ovarian cells.