Premium
Effects of the deficiency of the rhodanese‐like protein RhdA in Azotobacter vinelandii
Author(s) -
Cereda Angelo,
Carpen Aristodemo,
Picariello Gianluca,
Iriti Marcello,
Faoro Franco,
Ferranti Pasquale,
Pagani Silvia
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.03.028
Subject(s) - azotobacter vinelandii , rhodanese , polyhydroxybutyrate , aconitase , operon , mutant , biochemistry , enzyme , chemistry , biology , gene , bacteria , genetics , nitrogenase , nitrogen fixation
In Azotobacter vinelandii the rhdA gene codes for a protein (RhdA) of the rhodanese‐homology superfamily. By combining proteomics, enzymic profiles and ultrastructural observations, the phenotype of an A. vinelandii rhdA mutant was analyzed. We found that the A. vinelandii rhdA mutant, and not the wild‐type strain, accumulated polyhydroxybutyrate. RhdA deficiency enhanced the expression of enzymes of the polyhydroxybutyrate biosynthetic operon, and affected the activity of specific tricarboxylic acid cycle enzymes. The effect was dramatic on aconitase, in spite of comparable expression of aconitase polypeptides in both strains. By using a model system, we found that RhdA triggered protection from oxidants.