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Fluctuation of the SP/non‐SP phenotype in the C6 glioma cell line
Author(s) -
Platet Nadine,
Mayol Jean-François,
Berger François,
Hérodin Francis,
Wion Didier
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.02.071
Subject(s) - phenotype , cell culture , glioma , biology , cell , microbiology and biotechnology , chemistry , cancer research , gene , genetics
Using the C6 glioma cell as a paradigm, we found that (i) the clonogenicity of C6 cells is several orders of magnitude higher than the percentage of SP cells; (ii) non‐SP cells are able to generate SP cells, and conversely SP cells generate non‐SP cells; (iii) non‐SP sorted cells behave as tumorigenic cells. Hence, in C6 cells cultured in serum‐containing medium, SP cells can be generated from non‐SP cells. This dynamic equilibrium explains in C6 cells the maintenance of the SP phenotype with cell passaging and demonstrates the existence of tumorigenic non‐SP cells.