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Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion
Author(s) -
Fang Ji-Kang,
Prabu Subbuswamy K.,
Sepuri Naresh B.,
Raza Haider,
Anandatheerthavarada Hindupur K.,
Galati Domenico,
Spear Joseph,
Avadhani Narayan G.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.02.042
Subject(s) - protein subunit , phosphorylation , cytochrome c oxidase , intermembrane space , chemistry , biochemistry , oxidase test , microbiology and biotechnology , mitochondrion , biology , enzyme , bacterial outer membrane , gene , escherichia coli
We have mapped the sites of ischemia/reperfusion‐induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano‐LC–MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.