Premium
Neurogenin 3 recruits CBP co‐activator to facilitate histone H3/H4 acetylation in the target gene INSM1
Author(s) -
Breslin Mary B.,
Wang Hong-Wei,
Pierce Amy,
Aucoin Rebecca,
Lan Michael S.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.087
Subject(s) - acetylation , histone , histone h4 , activator (genetics) , histone h3 , chemistry , microbiology and biotechnology , gene , biology , biochemistry
INSM1 is a downstream target gene of neurogenin 3 (ngn3). A promoter construct containing the −426/+40 bp region transiently co‐transfected into NIH‐3T3 cells with a ngn3 expression plasmid resulted in a 12‐fold increase in promoter activity. The ngn3/E47 heterodimer selectively binds and activates the E‐box3 of the INSM1 promoter. The endogenous ngn3 and CREB‐binding protein (CBP) co‐activator occupy the INSM1 promoter, resulting in hyper‐acetylation of histone H3/H4 chromatin in a human neuroblastoma cell line, IMR‐32. Additionally, adenoviral ngn3 can induce endogenous INSM‐1 expression in pancreatic ductal carcinoma‐1 cells through the recruitment of CBP to the INSM1 promoter and increase the acetylation of the INSM1 promoter region.