Premium
Overexpression of mitochondrial uncoupling protein‐3 does not decrease production of the reactive oxygen species, elevated by palmitate in skeletal muscle cells
Author(s) -
Duval Carine,
Cámara Yolanda,
Hondares Elayne,
Sibille Brigitte,
Villarroya Francesc
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.085
Subject(s) - ucp3 , uncoupling protein , reactive oxygen species , mitochondrial ros , myogenesis , skeletal muscle , microbiology and biotechnology , mitochondrion , biology , biochemistry , myocyte , chemistry , endocrinology , adipose tissue , brown adipose tissue
Fatty acids induced an increase in reactive oxygen species (ROS) and enhanced NF‐κB activation in L6 myotubes differentiated in culture. Palmitate proved more effective than oleate in eliciting these effects. The induction of uncoupling protein‐3 (UCP3) at levels similar to those occurring in vivo, attained through the use of an adenoviral vector, led to a reduction of mitochondrial membrane potential in L6 myotubes. However, the capacity of palmitate to increase ROS was not reduced but, quite the opposite, it was moderately enhanced due to the presence of UCP3. The presence of UCP3 in mitochondria did not modify the expression of genes encoding ROS‐related enzymes, either in basal conditions or in the presence of palmitate. However, in the presence of UCP3, UCP2 mRNA expression was down‐regulated in response to palmitate. We conclude that UCP3 does not act as a protective agent against palmitate‐dependent induction of ROS production in differentiated skeletal muscle cells.