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Abl‐SH3 binding protein 2, 3BP2, interacts with CIN85 and HIP‐55
Author(s) -
Le Bras Séverine,
Moon Cheol,
Foucault Isabelle,
Breittmayer Jean-Philippe,
Deckert Marcel
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.084
Subject(s) - syk , sh3 domain , signal transducing adaptor protein , endocytic cycle , proto oncogene tyrosine protein kinase src , microbiology and biotechnology , sh2 domain , scaffold protein , biology , cell , kinase , phosphorylation , tyrosine kinase , signal transduction , endocytosis , biochemistry
The adapter 3BP2 is involved in leukocyte signaling downstream Src/Syk‐kinases coupled immunoreceptors. Here, we show that 3BP2 directly interacts with the endocytic scaffold protein CIN85 and the actin‐binding protein HIP‐55. 3BP2 co‐localized with CIN85 and HIP‐55 in T cell rafts and at the T cell/APC synapse, an active zone of receptors and proteins recycling. A binding region of CIN85 SH3 domains on 3BP2 was mapped to a PVPTPR motif in the first proline‐rich region of 3BP2, whereas the C‐terminal SH3 domain of HIP‐55 bound a more distal proline‐rich domain of 3BP2. Together, our data suggest an unexpected role of 3BP2 in endocytic and cytoskeletal regulation through its interaction with CIN85 and HIP‐55.