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Identification and characterization of microRNAs from the bovine adipose tissue and mammary gland
Author(s) -
Gu Zhiliang,
Eleswarapu Satyanaryana,
Jiang Honglin
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.081
Subject(s) - adipose tissue , microrna , biology , mammary gland , cloning (programming) , homologous chromosome , gene , positional cloning , genetics , microbiology and biotechnology , endocrinology , phenotype , cancer , breast cancer , computer science , programming language
We report the identification of bovine miRNAs by cloning small RNAs from adipose tissue and the mammary gland. Fifty‐nine distinct miRNAs were identified, five of them were not homologous to known mammalian miRNAs, and many of them had 3′ and/or 5′ end variants. Ribonuclease protection assays indicated that miR‐23a and miR‐24, whose genes are closely located on the same chromosome, were co‐expressed in different tissues. The assays also suggested a role for several miRNAs in the mammary gland and a role for miR‐133, a previously known skeletal and cardiac muscle‐specific miRNA, in the rumen, an organ unique to the ruminant.