Premium
Proteasome function is required for activation of programmed cell death in heat shocked tobacco Bright‐Yellow 2 cells
Author(s) -
Vacca Rosa A.,
Valenti Daniela,
Bobba Antonella,
de Pinto Maria C.,
Merafina Riccardo S.,
De Gara Laura,
Passarella Salvatore,
Marra Ersilia
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.071
Subject(s) - mg132 , proteasome , programmed cell death , microbiology and biotechnology , caspase , proteases , cytochrome c , proteasome inhibitor , reactive oxygen species , apoptosis , protease , chemistry , heat shock , biochemistry , heat shock protein , biology , enzyme , gene
To find out whether and how proteasome is involved in plant programmed cell death (PCD) we measured proteasome function in tobacco cells undergoing PCD as a result of heat shock (HS‐PCD). Reactive oxygen species (ROS) production, cytochrome c levels and caspase‐3‐like protease activation were also measured in the absence or presence of MG132, a proteasome inhibitor. We show that proteasome activation occurs in early phase of HS‐PCD upstream of the caspase‐like proteases activation; moreover inhibition of proteasome function by MG132 results in prevention of PCD perhaps due to the prevention of ROS production, cytochrome c release and caspase‐3‐like protease activation.