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p14 ARF interacts with N‐Myc and inhibits its transcriptional activity
Author(s) -
Amente Stefano,
Gargano Barbara,
Diolaiti Daniel,
Della Valle Giuliano,
Lania Luigi,
Majello Barbara
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.062
Subject(s) - chemistry , microbiology and biotechnology , biophysics , biology
In this study, we report that the human p14 ARF associates in vivo with the N‐Myc and inhibits N‐Myc mediated transcriptional activation. We have determined that the region (aa 140–300) encompassing the N‐Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK‐N‐BE neuroblastoma cell line p14 ARF over‐expression delocalized N‐Myc from the nucleoplasm into nucleoli and that N‐Myc regions required for interaction with p14 ARF are also important for nucleoli co‐localization. Finally, we determine that the N‐terminal region of the p14 ARF protein is involved in binding to c‐Myc and N‐Myc proteins.