Systematic screening of LNA/2′‐ O ‐methyl chimeric derivatives of a TAR RNA aptamer | Zendy

Di Primo Carmelo | Zendy; Rudloff Ivo | Zendy; Reigadas Sandrine | Zendy; Arzumanov Andrey A. | Zendy; Gait Michael J. | Zendy; Toulmé Jean-Jacques | Zendy

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Systematic screening of LNA/2′‐ O ‐methyl chimeric derivatives of a TAR RNA aptamer

Author(s) -

Di Primo Carmelo,

Rudloff Ivo,

Reigadas Sandrine,

Arzumanov Andrey A.,

Gait Michael J.,

Toulmé Jean-Jacques

Publication year - 2007

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2007.01.047

Subject(s) - aptamer , tar (computing) , rna , chemistry , surface plasmon resonance , nucleoside , hiv long terminal repeat , locked nucleic acid , microbiology and biotechnology , stereochemistry , biochemistry , biology , nanoparticle , nanotechnology , gene expression , materials science , gene , computer science , programming language , long terminal repeat

We synthesized and evaluated by surface plasmon resonance 64 LNA/2′‐ O ‐methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6‐nt loop of the TAR element of HIV‐1. Three combinations of LNA/2′‐ O ‐methyl nucleoside analogues where one or two LNA units are located on the 3′ side of the aptamer loop display an affinity for TAR below 1 nM, i.e. one order of magnitude higher than the parent RNA aptamer. One of these combinations inhibits the TAR‐dependent luciferase expression in a cell assay.

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