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Involvement of membrane protein GDE2 in retinoic acid‐induced neurite formation in Neuro2A cells
Author(s) -
Yanaka Noriyuki,
Nogusa Yoshihito,
Fujioka Yuichiro,
Yamashita Yosuke,
Kato Norihisa
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.035
Subject(s) - retinoic acid , neurite , chemistry , microbiology and biotechnology , membrane protein , membrane , biophysics , biochemistry , biology , in vitro , gene
We show that a glycerophosphodiester phosphodiesterase homolog, GDE2, is widely expressed in brain tissues including primary neurons, and that the expression of GDE2 in neuroblastoma Neuro2A cells is significantly upregulated during neuronal differentiation by retinoic acid (RA) treatment. Stable expression of GDE2 resulted in neurite formation in the absence of RA, and GDE2 accumulated at the regions of perinuclear and growth cones in Neuro2A cells. Furthermore, a loss‐of‐function of GDE2 in Neuro2A cells by RNAi blocked RA‐induced neurite formation. These results demonstrate that GDE2 expression during neuronal differentiation plays an important role for growing neurites.