RNA interference‐mediated silencing of the bursicon gene induces defects in wing expansion of silkworm | Zendy

Huang Jianhua | Zendy; Zhang Yong | Zendy; Li Minghui | Zendy; Wang Sibao | Zendy; Liu Wenbin | Zendy; Couble Pierre | Zendy; Zhao Guoping | Zendy; Huang Yongping | Zendy

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RNA interference‐mediated silencing of the bursicon gene induces defects in wing expansion of silkworm

Author(s) -

Huang Jianhua,

Zhang Yong,

Li Minghui,

Wang Sibao,

Liu Wenbin,

Couble Pierre,

Zhao Guoping,

Huang Yongping

Publication year - 2007

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2007.01.034

Subject(s) - rna interference , bombyx mori , gene silencing , biology , gene expression , gene , messenger rna , rna , wing , small interfering rna , microbiology and biotechnology , genetics , engineering , aerospace engineering

We studied the role of the bursicon gene in wing expansion. First, we investigated its expression at different developmental stages in the silkworm, Bombyx mori . Bursicon gene was expressed at low levels in larvae, high levels in pupae, and low levels again in adults. Then, we injected the double‐stranded bursicon RNA into B. mori pupae to test RNA interference. The level of bursicon mRNA was reduced significantly in pupae, and a deficit in wing expansion was observed in adults. In addition, the differential display reverse transcription polymerase chain reaction (DD‐RT‐PCR) was used to reveal differences in the expression of transcripts in response to the inhibition of bursicon. In conclusion, bursicon plays a key role in the stereotyped behavioral program involved in wing expansion.

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