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Glycogen synthesis by the direct or indirect pathways depends on glucose availability: In vivo studies in frog oocytes
Author(s) -
Preller Ana,
Kessi Eduardo,
Ureta Tito
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.029
Subject(s) - glycogen , in vivo , intracellular , chemistry , glycogen synthase , biochemistry , biophysics , biology , microbiology and biotechnology
Besides the classic direct route, frog oocytes incorporate glucosyl units into glycogen by the so‐called indirect pathway. The operation of both pathways depends on glucose availability. Below 0.5 mM glucose (calculated intracellular concentration), the indirect route accounts for 90% of polysaccharide formation, while the direct pathway supports 70% of total glucose incorporation when administered glucose is above 1.5 mM. A sigmoidal curve was obtained for the direct pathway with n H = 2.04, and half saturation was reached at 2.6 mM glucose. The curve for the indirect route presented an n H of 1.15 and an S 0.5 of 0.9 mM glucose.