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Regulation of Foxo‐1 and the angiopoietin‐2/Tie2 system by shear stress
Author(s) -
Chlench Sven,
Mecha Disassa Nigussie,
Hohberg Margret,
Hoffmann Christian,
Pohlkamp Theresa,
Beyer Gabriele,
Bongrazio Mauro,
Da Silva-Azevedo Luis,
Baum Oliver,
Pries Axel Radlach,
Zakrzewicz Andreas
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.028
Subject(s) - angiogenesis , phosphorylation , protein kinase b , angiopoietin receptor , angiopoietin , shear stress , chemistry , downregulation and upregulation , microbiology and biotechnology , foxo1 , enos , transcription factor , cancer research , biology , vascular endothelial growth factor , gene , vegf receptors , biochemistry , materials science , nitric oxide synthase , composite material , enzyme
Transcription factor Foxo‐1 can be inactivated via Akt‐mediated phosphorylation. Since shear stress activates Akt, we determined whether Foxo‐1 and the Foxo‐1‐dependent, angiogenesis‐related Ang‐2/Tie2‐system are influenced by shear stress in endothelial cells. Expression of Foxo‐1 and its target genes p27Kip1 and Ang‐2 was decreased under shear stress (6 dyn/cm 2 , 24 h), nuclear exclusion of Foxo‐1 by phosphorylation increased. eNOS and Tie2 were upregulated. No effects on Ang‐1 expression were detected. In conclusion, Foxo‐1 and Ang‐2/Tie2 are part of the molecular response to shear stress, which may regulate angiogenesis.