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Metabolism of glutathione in tumour cells as evidenced by 1H MRS
Author(s) -
Grande S.,
Luciani A.M.,
Rosi A.,
Palma A.,
Giovannini C.,
Sapora O.,
Guidoni L.,
Viti V.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.025
Subject(s) - glutathione , buthionine sulfoximine , metabolism , cysteine , glutathione synthetase , enzyme , glutamate receptor , cell growth , chemistry , biochemistry , glioma , cell culture , microbiology and biotechnology , biology , cancer research , genetics , receptor
1H MRS signals of glutathione and of free glutamate were examined in samples from cultured tumour cells, namely MCF‐7 from mammary carcinoma and TG98 from malignant glioma, with the aim of relating signal intensities to aspects of GSH metabolism. Spectra of cells harvested at different cell densities suggest that GSH and glu signal intensities are related to cell density and proliferation and their ratio is dependent on the activity of the γ‐glutamyl cysteine synthetase. The hypothesis is confirmed by experiments performed on cells treated with buthionine sulfoximine that inhibits the enzyme activity.