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Uncoupling of 3T3‐L1 gene expression from lipid accumulation during adipogenesis
Author(s) -
Temple Karla A.,
Basko Xheni,
Allison Margaret B.,
Brady Matthew J.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.01.007
Subject(s) - lipogenesis , adipogenesis , adipocyte , triglyceride , lipid droplet , 3t3 l1 , medicine , endocrinology , chemistry , glucose uptake , lipid metabolism , gene expression , extracellular , carbohydrate metabolism , biochemistry , biology , insulin , adipose tissue , gene , cholesterol
Adipocyte differentiation comprises altered gene expression and increased triglyceride storage. To investigate the interdependency of these two events, 3T3‐L1 cells were differentiated in the presence of glucose or pyruvate. All adipocytic proteins examined were similarly increased between the two conditions. In contrast, 3T3‐L1 adipocytes differentiated with glucose exhibited significant lipid accumulation, which was largely suppressed in the presence of pyruvate. Subsequent addition of glucose to the latter cells restored lipid accumulation and acute rates of insulin‐stimulated lipogenesis. These data indicate that extracellular energy is required for induction of adipocytic proteins, while only glucose sustained the parallel increase in triglyceride storage.